Protein Expression Eukaryotic cells HeLa R19

Get tips on using Prestained Protein Ladder (3.5 - 245 kDa) to perform Protein Ladder Prestained

Get tips on using BriteRuler - Pre-stained Protein Ladder (ab286875) to perform Protein Ladder Prestained

Get tips on using Pierce™ Prestained Protein MW Marker to perform Protein Ladder Prestained

Get tips on using PageRuler™ Prestained NIR Protein Ladder to perform Protein Ladder Prestained

Get tips on using BenchMark™ Pre-stained Protein Ladder to perform Protein Ladder Prestained

Get tips on using Anti-LC3A/B antibody to perform Autophagy assay cell type - HeLa

Get tips on using Atg7 (D12B11) Rabbit mAb to perform Autophagy assay cell type - HeLa

Get tips on using Micro BCA™ Protein Assay Kit to perform Protein quantification Colorimetric method

Get tips on using Pierce™ BCA Protein Assay Kit to perform Protein quantification Colorimetric method

Generally it has been difficult to isolate high-quality RNA from yeast because of problems disrupting the cells. Use of enzymes to disrupt cell wall can alter gene expression profiles. Therefore, physical disruption can result in high quality RNA for all downstream processing. Use of DNAse and proteinase K will remove traces of DNA contamination and proteins respectively.