RNA isolation / purification Yeast - Saccharomyces cerevisiae

Generally it has been difficult to isolate high-quality RNA from yeast because of problems disrupting the cells. Use of enzymes to disrupt cell wall can alter gene expression profiles. Therefore, physical disruption can result in high quality RNA for all downstream processing. Use of DNAse and proteinase K will remove traces of DNA contamination and proteins respectively.

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Found 2 matching solutions for this experiment

Upstream tips
Samples can be flash frozen (-80C) before use, this will likely cause lower RNA yield.
Downstream tips
When checking the yield by means of a spectrophotometer, use the supplied TE buffer to zero the machine
MicroRNA isolation kit

A&A Biotechnology

Protocol tips
MicroRNA isolation kit (A&A Biotechnology) following the manufacturers’ protocol
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