Isolating DNA from tissues and paraffin-embedded tissue samples can be challenging as double-stranded DNA is physically fragile and highly susceptible to exo- and endonucleases. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in the presence of DNAse inhibitors. Further, extracting DNA from the nucleus need specific methods by combining physical, mechanical and chemical lysis approaches,
Get tips on using In Situ Cell Death Detection Kit, Fluorescein to perform TUNEL assay cell type - A549, NCI-H460, H1299 human lung cancer cells
Get tips on using in situ Cell Death Detection Kit, POD to perform TUNEL assay cell type - A549, NCI-H460, H1299 human lung cancer cells
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Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary rat marrow stromal cells
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Get tips on using RiboPure™ RNA Purification Kit, bacteria to perform
Get tips on using RiboPure™ RNA Purification Kit, bacteria to perform
Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary human airway epithelial cells
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