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Get tips on using Maxwell® 16 LEV simplyRNA Purification Kit to perform RNA isolation / purification Cells - immortalized A549

Get tips on using High Pure RNA Isolation Kit to perform RNA isolation / purification Tissue - Rat Liver

Get tips on using High Pure RNA Isolation Kit to perform RNA isolation / purification Tissue - Rat Kidney

Get tips on using PicoPure™ RNA Isolation Kit to perform RNA isolation / purification Tissue - Rat Bronchi

Get tips on using FuGENE® 6 Transfection Reagent to perform DNA transfection Mammalian cells - Primary cells Rat aortic smooth muscle cells (rASMC)

Get tips on using NucleoSpin® RNA to perform RNA isolation / purification Cells - immortalized MIA PaCa-2

Get tips on using PureZOL™ RNA Isolation Reagent to perform RNA isolation / purification Tissue - Rat Blood / Serum / Plasma / Buffy coat

Protein isolation is a technique that involves isolation and/ or purification of protein from cells or tissues via chromatography or electrophoresis. The major challenges in protein isolation include: 1. The concentration of proteins in cells is variable and tends to be small for some intracellular proteins. Unlike nucleic acids, proteins cannot be amplified. 2. Proteins are more unstable than nucleic acids. They are easily denatured under suboptimal temperature, pH or salt concentrations. 3. Finally, no generalized technique/protocol can be applied for protein isolation. Proteins may have different electrostatic (number of positively or negatively charged amino acids) or hydrophobic properties. Therefore, protein purification requires multiple steps depending on their charge (a negatively charged resin/column for positively charged proteins and vice-versa), dissolution (using detergents) and unlike in the case of DNA and RNA, instead of using salts, proteins should be isolated by isoelectric precipitation.

Get tips on using Lipofectamine® 2000 Transfection Reagent to perform DNA transfection Mammalian cells - Primary cells Rat dermal fibroblasts (rDF)

Get tips on using X-tremeGENE™ HP DNA Transfection Reagent to perform DNA transfection Mammalian cells - Primary cells Rat astrocytes