shRNA gene silencing Rat WKY

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

Get tips on using Rab 7 siRNA (h) to perform siRNA / miRNA gene silencing Human - BEAS-2B RAB7A

Get tips on using Rab 5C siRNA (h) to perform siRNA / miRNA gene silencing Human - BEAS-2B RAB5C

Get tips on using Stealth siRNA_GATA2 to perform siRNA / miRNA gene silencing Human - LAD2 GATA2

Get tips on using KRAS siRNA to perform siRNA / miRNA gene silencing Human - A431 KRAS

Get tips on using STEAP1 siRNA to perform siRNA / miRNA gene silencing Human - LNCap STEAP1

Get tips on using siRNA s2748 to perform siRNA / miRNA gene silencing Human - HeLa Cdc20

Get tips on using siRNA SKIL to perform siRNA / miRNA gene silencing Human - RWPE SKIL