Protein expression and purification Mammalian cells HeLa

Get tips on using PL_MmBax to perform Protein Expression Eukaryotic cells - CHO Bax

Get tips on using PL_MmBak1 to perform Protein Expression Eukaryotic cells - CHO Bak1

Get tips on using PL_MmAtf4 to perform Protein Expression Eukaryotic cells - CHO Atf4

Get tips on using CpGfree to perform Protein Expression Eukaryotic cells - CHO EGFP

Get tips on using CpGrich to perform Protein Expression Eukaryotic cells - CHO EGFP

Get tips on using AllPrep RNA/Protein Kit to perform RNA isolation / purification Cells - primary human epidermal keratinocytes

Get tips on using Live and Dead Cell Assay (Abcam) to perform Live / Dead assay mammalian cells - MC3T3

Get tips on using Live and Dead Cell Assay (Abcam) to perform Live / Dead assay mammalian cells - rabbit bone marrow mesenchymal stem cells

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.

Get tips on using RIPA Buffer (10X) to perform Protein isolation Mammalian cells - Human gingival epithelial cells