siRNA / RNAi /miRNA transfection Human Cells THP-1

Get tips on using CD74 siRNA and shRNA Plasmids (h) to perform siRNA / miRNA gene silencing Human - HT-1376 CD74

Get tips on using Live/Dead Double Staining Kit (Merck) to perform Live / Dead assay mammalian cells - THP-1

Get tips on using SignalSilence® SAPK/JNK siRNA to perform siRNA / miRNA gene silencing Human - KGN SAPK/JNK

Get tips on using pcDNA™3.1 (+) Mammalian Expression Vector to perform siRNA / miRNA gene silencing Human - U251 cofilin-1 (CFL1)

Get tips on using Human MCP-1 / CCL2 PicoKine™ ELISA Kit to perform ELISA Human - MCP1

Get tips on using Human IGF-I/IGF-1 Quantikine ELISA Kit to perform ELISA Human - IGF-I

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.

Get tips on using Arp2 siRNA (h) to perform siRNA / miRNA gene silencing Human - T47-D Arp-2

Get tips on using Control siRNA-A to perform siRNA / miRNA gene silencing Human - OV2008 Yap Gene Lipofectamine

Get tips on using YAP siRNA (h) to perform siRNA / miRNA gene silencing Human - OV2008 Yap Gene Lipofectamine