siRNA / miRNA gene silencing Human HeLa EPAS-1

Get tips on using ON-TARGETplus Mouse Sirt2 (64383) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Mouse - RAW264.7 Sirt2

The RNA interference (RNAi) is used to inhibit gene expression or translation, by neutralizing targeted mRNA molecules. Two types of RNA molecules such as microRNA (miRNA) and small interfering RNA (siRNA) play a central role in RNAi. Few points have to considered to increase the transfection efficiency of siRNA. Always use healthy, actively dividing cells to maximize transfection efficiency. The confluency of cells should be between 50-70%. Always use the most appropriate siRNA concentration to avoid off-target effects and unwanted toxic side effects. Positive and negative controls should be used for each and every experiment to determine transfection efficiency.

Get tips on using Silencer® Select- Gdf10 siRNA to perform siRNA / miRNA gene silencing Mouse - 3T3-L1 BMP-3b/GDF10

Get tips on using C/EBP β siRNA (r) to perform siRNA / miRNA gene silencing Rat - Glial cells C/EBP‐β

Get tips on using FlexiTube GeneSolution GS26354 for GNL3 to perform siRNA / miRNA gene silencing Human - MDA-MB-231 GNL3

Get tips on using ON-TARGETplus Rat Arhgef7 (114559) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Rat - MTLn3 β-Pix/Arhgef7

Get tips on using ON-TARGETplus Mouse Hspa5 (14828) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Mouse - MC3T3-E1 Grp78/Hspa5

Get tips on using siGENOME Rat Gata1 (25172) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Rat - C6 (rat glioma) Gata1

Get tips on using Accell Mouse Nrep (27528) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Mouse - 3T3-L1 P311/Nrep

Get tips on using Lipofectamine® 2000 Transfection Reagent to perform siRNA / RNAi /miRNA transfection Human Cells - HeLa Lipofectamine