RNA

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The RNA interference (RNAi) is used to inhibit gene expression or translation, by neutralizing targeted mRNA molecules. Two types of RNA molecules such as microRNA (miRNA) and small interfering RNA (siRNA) play a central role in RNAi. Few points have to considered to increase the transfection efficiency of siRNA. Always use healthy, actively dividing cells to maximize transfection efficiency. The confluency of cells should be between 50-70%. Always use the most appropriate siRNA concentration to avoid off-target effects and unwanted toxic side effects. Positive and negative controls should be used for each and every experiment to determine transfection efficiency.

RNA siRNA / RNAi /miRNA transfection Rat H9c2 Cationic and neutral lipids

The RNA interference (RNAi) is used to inhibit gene expression or translation, by neutralizing targeted mRNA molecules. Two types of RNA molecules such as microRNA (miRNA) and small interfering RNA (siRNA) play a central role in RNAi. Few points have to considered to increase the transfection efficiency of siRNA. Always use healthy, actively dividing cells to maximize transfection efficiency. The confluency of cells should be between 50-70%. Always use the most appropriate siRNA concentration to avoid off-target effects and unwanted toxic side effects. Positive and negative controls should be used for each and every experiment to determine transfection efficiency.

RNA siRNA / RNAi /miRNA transfection Rat A-10 Cationic lipid based

Get tips on using Amino Allyl MessageAmp™ II aRNA Amplification Kit to perform RNA amplification & labeling Fish - Total RNA, Fundulus heteroclitus Cyanine 3 & 5

Products Thermo Fisher Scientific Amino Allyl MessageAmp™ II aRNA Amplification Kit

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Tissue - Rat Spleen

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Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Tissue - Rat Lung

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Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Tissue - Rat Liver

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Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Tissue - Rat Heart

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Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Tissue - Rat Brain

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Get tips on using RNeasy Micro Kit to perform RNA isolation / purification Tissue - Rat Uterus

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Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Tissue - Rat estes

Products Qiagen RNeasy Mini Kit

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