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|Do not add antibiotics to media during transfection; this causes cell death.|
|Mix all the complex reactions and incubate the cells at 37°C in a CO2 incubator for 4 hours.
Add 50 µL of growth medium containing 3X the normal concentration of serum without removing the transfection mixture and assay for gene activity at 24–72 hours post-transfection.
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