Western blot Biotin -NA- -NA-

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RNA isolation / purification Cells - primary bovine pulmonary artery smooth muscle cells Experiment

When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.

RNA RNA isolation / purification Cells primary bovine pulmonary artery smooth muscle cells

RNA isolation / purification Cells - primary bovine umbilical vein endothelial cells Experiment

RNA RNA isolation / purification Cells primary bovine umbilical vein endothelial cells

RNA isolation / purification Bacteria - Gram positive Clostridum botulinum Experiment

The biggest problem in isolating RNA from gram-positive bacteria is the disruption of the cell wall. A lot of protocols employ enzymatic digestion (pretreatment) which may affect gene expression patterns of certain genes. Therefore physical disruption using beads can be a best alternative.

RNA RNA isolation / purification Bacteria Gram positive Clostridum botulinum

TRIzol Reagent Product

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary bovine aortic endothelial cells