Protein expression and purification Yeast

Get tips on using Pierce™ Unstained Protein MW Marker to perform Protein Ladder Unstained

Get tips on using Biotinylated Protein Ladder Detection Pack #7727 to perform Protein Ladder Immunofluorescence

Get tips on using Tau Protein Ladder, 6 isoforms human to perform Protein Ladder Immunofluorescence

Get tips on using Prestained Protein Ladder (3.5 - 245 kDa) to perform Protein Ladder Prestained

Get tips on using BriteRuler - Pre-stained Protein Ladder (ab286875) to perform Protein Ladder Prestained

Get tips on using Pierce™ Prestained Protein MW Marker to perform Protein Ladder Prestained

Get tips on using PageRuler™ Prestained NIR Protein Ladder to perform Protein Ladder Prestained

Get tips on using BenchMark™ Pre-stained Protein Ladder to perform Protein Ladder Prestained

Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase

The biggest problem in isolating RNA from gram-positive bacteria is the disruption of the cell wall. A lot of protocols employ enzymatic digestion (pretreatment) which may affect gene expression patterns of certain genes. Therefore physical disruption using beads can be a best alternative.