Reporter gene assay luciferase - HepG2 and Huh7 cells

Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase

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5 years ago

5 years ago by J.Han China

Which transfection reagent would work better on stem cells?

I am planning on running reporter assays on stem cells and I would like to know which transfection reagent would work better?

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Luciferase activity was assayed using SuperLightTM Luciferase Reporter Gene Assay and measured using a Spectra Max M3 microplate reader (Molecular Devices, Inc., Sunnyvale, CA).
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