siRNA / miRNA gene silencing Human Primary Human Aortic Endothelial Cells GLO-1

Get tips on using ROS-Glo™ H2O2 Assay to perform ROS assay cell type - PANC-, BxPC-3 human pancreas

Get tips on using ROS-Glo™ H2O2 Assay to perform ROS assay cell type - BEAS-2B human bronchial epithelial cell line

Get tips on using siGENOME Rat Nrp1 siRNA to perform siRNA / miRNA gene silencing Rat - Schwann cells Nrp1

Get tips on using FlexiTube GeneSolution GS7052 for TGM2 to perform siRNA / miRNA gene silencing Human - Caki-2 TGM2

Get tips on using Human NRG1-beta 1/HRG1-beta 1 DuoSet ELISA to perform ELISA Human - NRG1

Get tips on using KIM-1 (human) ELISA kit to perform ELISA Human - KIM-1

Get tips on using HO-1 (human), ELISA kit to perform ELISA Human - HO-1

Get tips on using Beta-Glo® Assay System to perform Reporter gene assay β-galactosidase substrates - rat mesenchymal stem cells (MSCs)

Get tips on using Purified Mouse Anti-Human ZO-1 Clone 1/ZO-1 (RUO) to perform Western blotting ZO-1

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.