siRNA / miRNA gene silencing Human Primary Human Aortic Endothelial Cells GLO-1

Get tips on using Human HO 1 ELISA Kit (ab133064) to perform ELISA Human - HO-1

Get tips on using Human Dkk-1 ELISA Kit (RAB0143) to perform ELISA Human - Dkk-1

Get tips on using Human Dkk-1 DuoSet ELISA (DY1906) to perform ELISA Human - Dkk-1

Get tips on using Brn-3b siRNA (m) to perform siRNA / miRNA gene silencing Rat - Retinal stem cells Brn-3b

Get tips on using Human ICAM-1/CD54 Antibody to perform Western blotting ICAM-1

Get tips on using ON-TARGETplus Mouse Jun siRNA to perform siRNA / miRNA gene silencing Mouse - Embryonic stem cells Jun

Get tips on using ON-TARGETplus Mouse Gpam siRNA to perform siRNA / miRNA gene silencing Mouse - Embryonic stem cells Gpat1

Get tips on using RealTime-Glo™ MT Cell Viability Assay to perform Live / Dead assay mammalian cells - THP-1

An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

Get tips on using TransIT-TKO Transfection Reagent to perform siRNA / RNAi /miRNA transfection Human Cells - Primary splenocytes Polymer / lipid