Western blot Secondary Antibody Goat Mouse

Get tips on using PE anti-human CD126 (IL-6Rα) Antibody to perform Flow cytometry Anti-bodies Human - CD126/IL-6Ralpha

Get tips on using Anti-p62/SQSTM1 antibody produced in rabbit to perform Autophagy assay cell type - Hippocampal neural stem cells

Get tips on using Biotin Rat Anti-Mouse CD106 to perform Flow cytometry Anti-bodies Mouse - CD106/Vcam-1

Get tips on using Mouse ANGPTL3 ELISA to perform ELISA Mouse - Angiopoietin-Like 3 (AngptL3)

DNA-protein interactions are studied by using ChIP. The basic steps in this technique are crosslinking, sonication, immunoprecipitation, and analysis of the immunoprecipitated DNA. During ChIP, if chromatin is under-fragmented or fragments are too large which can lead to the increased background and lower resolution. Shorter cross-linking times (5-10 min) and/or lower formaldehyde concentrations (<1%) may improve shearing efficiency. If Chromatin is over-fragmented, then optimize shearing conditions for each cell type to improve ChIP efficiency. Over-sonication of chromatin may disrupt chromatin integrity and denature antibody epitopes. If you do not see any product or very little product in the input PCR reactions, add 5–10 μg chromatin per IP.

Get tips on using Anti-53BP1 (phospho S25) antibody, rabbit polyclonal to perform Immunohistochemistry 53BP2 phospho (ser-25) - Rabbit IgG Human -NA-

Get tips on using Donkey anti-mouse IgG to perform Immunohistochemistry Anti-mouse IgG - Donkey Mouse Rhodamin red

Get tips on using Anti-Glial Fibrillary Acidic Protein Antibody, clone GA5 to perform Immunohistochemistry Rat - GFAP

Get tips on using Phospho-PI3 Kinase p85 (Tyr458)/p55 (Tyr199) Antibody to perform Autophagy assay cell type - HepG2

Get tips on using Anti-PI 3 Kinase p85 alpha antibody [EP380Y] to perform Autophagy assay cell type - HepG2