siRNA / miRNA gene silencing Human SUIT-2

Get tips on using MicroRNA isolation kit to perform siRNA / miRNA gene silencing Rat - IEC-6 HuR

Get tips on using Human BMP-2 PicoKine™ ELISA Kit to perform ELISA Human - BMP-2

Get tips on using CD133/2 Antibody, anti-human, APC to perform Flow cytometry Anti-bodies Human - CD133

Get tips on using CD133/2 Antibody, anti-human, PE to perform Flow cytometry Anti-bodies Human - CD133

Get tips on using FlexiTube GeneSolution GS27279 for Tnfrsf12a to perform siRNA / miRNA gene silencing Mouse - B16-BL6 FN14/Tnfrsf12a

Get tips on using 3D-Gene® Mouse miRNA Oligo chip (ver.21) to perform Microarray Gene expression arrays - Mouse liver tissue Cyanine-3-CTP

Get tips on using Human Lipocalin-2 ELISA Kit (NGAL) (ab119600) to perform ELISA Human - NGAL/LCN2

Get tips on using Human Lipocalin-2/NGAL Quantikine ELISA Kit to perform ELISA Human - NGAL/LCN2

DNA-protein interactions are studied by using ChIP. The basic steps in this technique are crosslinking, sonication, immunoprecipitation, and analysis of the immunoprecipitated DNA. During ChIP, if chromatin is under-fragmented or fragments are too large which can lead to the increased background and lower resolution. Shorter cross-linking times (5-10 min) and/or lower formaldehyde concentrations (<1%) may improve shearing efficiency. If Chromatin is over-fragmented, then optimize shearing conditions for each cell type to improve ChIP efficiency. Over-sonication of chromatin may disrupt chromatin integrity and denature antibody epitopes. If you do not see any product or very little product in the input PCR reactions, add 5–10 μg chromatin per IP.