Get tips on using Mucin 1 Antibody (VU4H5): sc-7313 to perform Immunohistochemistry Human - Muc-1
Get tips on using Recombinant Anti-MUC1 antibody [EPR1023] (ab109185) to perform Immunohistochemistry Human - Muc-1
Get tips on using Sox-9 Antibody (E-9): sc-166505 to perform Immunohistochemistry Human - SOX9
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Get tips on using Notch 1 Antibody (A-8): sc-376403 to perform Immunohistochemistry Human - Notch1
Get tips on using Anti-Androgen Receptor antibody [AR 441] (ab9474) to perform Immunohistochemistry Human - AR
Get tips on using Anti-Villin antibody [3E5G11] - N-terminal (ab201989) to perform Immunohistochemistry Human - Villin
Get tips on using Mucin 16 Antibody (C-6): sc-365002 to perform Immunohistochemistry Human - CA125
Get tips on using Anti-Glutamine Synthetase Antibody, clone GS-6 to perform Immunohistochemistry Rat - GS
RNA-Seq is a method to sequence RNA by applying Next Generation Sequencing (NGS). The quality of RNA is critical for the success of RNA-Seq. The integrity of RNA is measured by the RNA integrity number (RIN). RIN is computed from RNA electrophoresis and electropherogram profiles (the peak area of the 28S rRNA should be approximately twice the peak area of the 18S rRNA). If you get the RIN value lower than 7, the possibility of getting the low quality of RNA-seq data is high. To get a high quality RNA, it is better to work with fresh samples or snap-freeze the tissues in liquid nitrogen as quickly as possible and store them at -80°C until further use. Make sure designated areas and all your filter tips, microfuge tubes, plastic, and glassware are RNase-free.
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