Protein expression and purification Insect cells

Get tips on using pPIC9-His-FIP-Ganoderma to perform Protein Expression Eukaryotic cells - P. pastoris Ganoderma FIP

Get tips on using MrNV-pGEX-6P-1 to perform Protein Expression Prokaryotic cells - E. coli MrNV capsid

Get tips on using pIRES2-EGFP-PBD-1 to perform Protein Expression Prokaryotic cells - E. coli PBD1-EGFP

Get tips on using pRSET A-FhFtn-1 to perform Protein Expression Prokaryotic cells - E. coli FhFtn-1

Get tips on using pTRAkc-AH/pRIC 3.0 to perform Protein Expression Prokaryotic cells - A. tumefaciens BFDV cp

Get tips on using pTRAkc-ERH/pRIC 3.0 to perform Protein Expression Prokaryotic cells - A. tumefaciens BFDV cp

Get tips on using pVL1392-RIP1 8-322-His to perform Protein Expression Eukaryotic cells - S. frugiperda RIP1

Get tips on using pIVEX-GAA-omega-PHACTR1-H to perform Protein Expression Eukaryotic cells - N. benthamiana PHACTR1

Get tips on using pHR-CMV-TetO2-VSV-G to perform Protein Expression Eukaryotic cells - HEK293 VSV-G

Protein ladders are a set of standards known as molecular weight proteins that are utilized to identify the approximate size of a protein molecule run on a PAGE gel electrophoresis. The challenges in running the ladders are the choice of appropriate protein standard as it is used as visual evidence of protein migration, transfer efficiency, and positive control. Suitable protein markers can be selected on the basis of required properties and applications, i.e., fluorescent ladder, IEF, 2D SDS-PAGE ladder, natural ladder with an isoelectric point, and optimized ladders for Western Blot chemiluminescence detection. The key factors for running a distinct protein ladder are buffer conditions, charge/voltage at migration time, and the gel's concentration.