Immunohistochemistry Anti-Glial Fibrillary Acidic Protein (GFAP) Mouse

Get tips on using CC10 Antibody (E-11): sc-365992 to perform Immunohistochemistry Human - SCGB1A1 /CC10

Get tips on using CC10 Antibody (B-6): sc-390313 to perform Immunohistochemistry Human - SCGB1A1 /CC10

Get tips on using FGF-10 Antibody (3C7): sc-293208 to perform Immunohistochemistry Human - FGF-10

Get tips on using Mucin 5AC Antibody (45M1): sc-21701 to perform Immunohistochemistry Human - Muc-5AC

Get tips on using Mucin 1 Antibody (VU4H5): sc-7313 to perform Immunohistochemistry Human - Muc-1

Get tips on using Sox-9 Antibody (E-9): sc-166505 to perform Immunohistochemistry Human - SOX9

Get tips on using Notch 1 Antibody (A-8): sc-376403 to perform Immunohistochemistry Human - Notch1

Get tips on using Mucin 16 Antibody (C-6): sc-365002 to perform Immunohistochemistry Human - CA125

Get tips on using Anti-p62/SQSTM1 antibody produced in rabbit to perform Autophagy assay cell type - Hippocampal neural stem cells

DNA-protein interactions are studied by using ChIP. The basic steps in this technique are crosslinking, sonication, immunoprecipitation, and analysis of the immunoprecipitated DNA. During ChIP, if chromatin is under-fragmented or fragments are too large which can lead to the increased background and lower resolution. Shorter cross-linking times (5-10 min) and/or lower formaldehyde concentrations (<1%) may improve shearing efficiency. If Chromatin is over-fragmented, then optimize shearing conditions for each cell type to improve ChIP efficiency. Over-sonication of chromatin may disrupt chromatin integrity and denature antibody epitopes. If you do not see any product or very little product in the input PCR reactions, add 5–10 μg chromatin per IP.