Immunohistochemistry Collagen VII antibody [LH7.2] Mouse

Get tips on using PE anti-human CD111 (Nectin-1) Antibody to perform Flow cytometry Anti-bodies Human - CD111/Nectin-1

Get tips on using PE anti-human CD126 (IL-6Rα) Antibody to perform Flow cytometry Anti-bodies Human - CD126/IL-6Ralpha

Get tips on using Anti-p62/SQSTM1 antibody produced in rabbit to perform Autophagy assay cell type - Hippocampal neural stem cells

Get tips on using Mouse ANGPTL3 ELISA to perform ELISA Mouse - Angiopoietin-Like 3 (AngptL3)

DNA-protein interactions are studied by using ChIP. The basic steps in this technique are crosslinking, sonication, immunoprecipitation, and analysis of the immunoprecipitated DNA. During ChIP, if chromatin is under-fragmented or fragments are too large which can lead to the increased background and lower resolution. Shorter cross-linking times (5-10 min) and/or lower formaldehyde concentrations (<1%) may improve shearing efficiency. If Chromatin is over-fragmented, then optimize shearing conditions for each cell type to improve ChIP efficiency. Over-sonication of chromatin may disrupt chromatin integrity and denature antibody epitopes. If you do not see any product or very little product in the input PCR reactions, add 5–10 μg chromatin per IP.

Get tips on using OxPhos Complex IV subunit IV Monoclonal Antibody (20E8C12) to perform Western blotting COX4

Get tips on using Anti-COX IV antibody - Mitochondrial Loading Control (ab16056) to perform Western blotting COX4

Get tips on using Recombinant Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab115730) to perform Western blotting GLUT1

Get tips on using Anti-Hsp60 antibody [Mab11-13] - Mitochondrial Marker (ab13532) to perform Western blotting Hsp60

Get tips on using Recombinant Anti-TAK1 (phospho S439) antibody [EPR2863] (ab109404) to perform Western blotting Tak1