siRNA / RNAi /miRNA transfection Human Cells OV-2008

Get tips on using Stealth siRNA_SPI1 to perform siRNA / miRNA gene silencing Human - LAD2 PU.1/SPI1

An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

Get tips on using Rn_Pdcd8_1 FlexiTube siRNA to perform siRNA / miRNA gene silencing Rat - H9c2 AIF/Pdcd8

Get tips on using Net siRNA (m) to perform siRNA / miRNA gene silencing Mouse - FL83B Elk3/Net

Get tips on using ATG5 siRNA (m) to perform siRNA / miRNA gene silencing Mouse - NIH-3T3 Atg5

Get tips on using p130 siRNA (m) to perform siRNA / miRNA gene silencing Mouse - mESC Rbl2/p130

Get tips on using MITF siRNA (m) to perform siRNA / miRNA gene silencing Mouse - B16-F10 MITF

Get tips on using TLR4 siRNA (m) to perform siRNA / miRNA gene silencing Mouse - Neuro 2a TLR4

Get tips on using C23 siRNA (m) to perform siRNA / miRNA gene silencing Mouse - Neuro 2a C23

Get tips on using ANT2 siRNA (m) to perform siRNA / miRNA gene silencing Mouse - 3T3-L1 ANT2