siRNA / miRNA gene silencing Human Min-6

Get tips on using FuGENE® 6 Transfection Reagent to perform DNA transfection Mammalian cells - Primary cells Rat articular chondrocytes

Get tips on using FuGENE® 6 Transfection Reagent to perform DNA transfection Mammalian cells - Primary cells Rat hepatic stellate cells

Get tips on using FuGENE® 6 Transfection Reagent to perform DNA transfection Mammalian cells - Primary cells Rat aortic smooth muscle cells (rASMC)

Get tips on using FuGENE® 6 Transfection Reagent to perform DNA transfection Mammalian cells - Primary cells Rat pulmonary artery smooth muscle cell (pPASMC)

Get tips on using ALCAM Antibody (B-6): sc-74558 to perform Immunohistochemistry Mouse - CD166 / ALCAM

Get tips on using Ni-NTA Fast Start Kit (6) to perform Protein tag Purification of His-tagged proteins

When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.

Get tips on using Anti-Glutamine Synthetase Antibody, clone GS-6 to perform Immunohistochemistry Rat - GS

Get tips on using NFκB p65 Antibody (F-6): sc-8008 to perform Immunohistochemistry Mouse - NFκB / p65

Get tips on using cyclin D1 Antibody (DCS-6): sc-20044 to perform Western blotting Cyclin D1