siRNA / miRNA gene silencing Mouse RGC-5

DNA microarrays enable researchers to monitor the expression of thousands of genes simultaneously. However, the sensitivity, accuracy, specificity, and reproducibility are major challenges for this technology. Cross-hybridization, combination with splice variants, is a prime source for the discrepancies in differential gene expression calls among various microarray platforms. Removing (either from production or downstream bioinformatic analysis) and/or redesigning the microarray probes prone to cross-hybridization is a reasonable strategy to increase the hybridization specificity and hence, the accuracy of the microarray measurements.

DNA microarrays enable researchers to monitor the expression of thousands of genes simultaneously. However, the sensitivity, accuracy, specificity, and reproducibility are major challenges for this technology. Cross-hybridization, combination with splice variants, is a prime source for the discrepancies in differential gene expression calls among various microarray platforms. Removing (either from production or downstream bioinformatic analysis) and/or redesigning the microarray probes prone to cross-hybridization is a reasonable strategy to increase the hybridization specificity and hence, the accuracy of the microarray measurements.

Get tips on using MboII (5 U/µL) to perform Restriction Enzymes MboII

Get tips on using TurboCapture 96 mRNA Kit (5) to perform mRNA / Ribonucleoprotein isolation / purification mRNA

Get tips on using TurboCapture 384 mRNA Kit (5) to perform mRNA / Ribonucleoprotein isolation / purification mRNA

Get tips on using GenomONE™-Neo HVJ-E Membrane Fusion Transfection Kit to perform siRNA / miRNA gene silencing Human - U937 MK2 (MAPK Kinase 2) Viral vectors

Get tips on using GenomONE™-Neo HVJ-E Membrane Fusion Transfection Kit to perform siRNA / miRNA gene silencing Human - Jurkat MK2 (MAPK Kinase 2) Viral vectors

Get tips on using MlsI (MscI) (5 U/µL) to perform Restriction Enzymes MlsI / MscI

Get tips on using Hin1II (NlaIII) (5 U/µL) to perform Restriction Enzymes Hin1II / NlaIII

Get tips on using MssI (PmeI) (5 U/µL) to perform Restriction Enzymes PmeI / MssI