siRNA / miRNA gene silencing Rat RGC-5

Get tips on using TurboCapture 384 mRNA Kit (5) to perform mRNA / Ribonucleoprotein isolation / purification mRNA

Get tips on using GenomONE™-Neo HVJ-E Membrane Fusion Transfection Kit to perform siRNA / miRNA gene silencing Human - U937 MK2 (MAPK Kinase 2) Viral vectors

Get tips on using GenomONE™-Neo HVJ-E Membrane Fusion Transfection Kit to perform siRNA / miRNA gene silencing Human - Jurkat MK2 (MAPK Kinase 2) Viral vectors

Get tips on using MlsI (MscI) (5 U/µL) to perform Restriction Enzymes MlsI / MscI

Get tips on using Hin1II (NlaIII) (5 U/µL) to perform Restriction Enzymes Hin1II / NlaIII

Get tips on using MssI (PmeI) (5 U/µL) to perform Restriction Enzymes PmeI / MssI

Get tips on using BfuI (BciVI) (5 U/µL) to perform Restriction Enzymes BciVI / BfuI

Get tips on using EndoFree Plasmid Mega Kit (5) to perform DNA isolation / purification Plasmid purification

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.

Get tips on using pIEX-5-6His-mMBP-UMODpXR to perform Protein Expression Eukaryotic cells - HEK293 mMBP