Get tips on using Anti-p62 (SQSTM1) (Human) pAb to perform Autophagy assay cell type - MEFs (mouse embryonic fibroblasts)
Get tips on using DeadEnd™ Colorimetric TUNEL System to perform TUNEL assay cell type - Mouse skeletal muscle cells
Get tips on using Anti-LC3B antibody produced in rabbit to perform Autophagy assay cell type - Mouse lung tissue
Get tips on using Anti-LC3B antibody produced in rabbit to perform Autophagy assay cell type - Mouse embryonic fibroblasts
Get tips on using CYTO-ID® Autophagy detection kit to perform Autophagy assay cell type - Mouse embryonic fibroblasts
Get tips on using TiterTACS™ Colorimetric Apoptosis Detection Kit to perform TUNEL assay cell type - Mouse endothelial cells
The RNA interference (RNAi) is used to inhibit gene expression or translation, by neutralizing targeted mRNA molecules. Two types of RNA molecules such as microRNA (miRNA) and small interfering RNA (siRNA) play a central role in RNAi. Few points have to considered to increase the transfection efficiency of siRNA. Always use healthy, actively dividing cells to maximize transfection efficiency. The confluency of cells should be between 50-70%. Always use the most appropriate siRNA concentration to avoid off-target effects and unwanted toxic side effects. Positive and negative controls should be used for each and every experiment to determine transfection efficiency.
Get tips on using Mouse/Rat/Porcine/Canine TGF-beta 1 Quantikine ELISA Kit to perform ELISA Mouse - TGF-beta 1
Get tips on using Mouse CRP / C Reactive Protein / PTX1 PicoKine™ ELISA Kit to perform ELISA Mouse - C-Reactive Protein/CRP
Acid phosphatase detection heavily relies on determining the concentration of tartrate-resistant acid phosphatase (TRAP) in the sample. Hence, sample preparation is very crucial and it should be done strictly as per kit manufacturer instructions to avoid any inconsistency and poor sensitivity
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