protein-expression-and-purification-mammalian-cells-hek-293-mt-pa

Get tips on using Live and Dead Cell Assay (Abcam) to perform Live / Dead assay mammalian cells - MC3T3

Get tips on using pAF3 to perform Protein Expression Prokaryotic cells - B. subtilis PHY US417

Get tips on using pASG-IBA35 to perform Protein Expression Prokaryotic cells - E. coli D1

Get tips on using pASG-IBA5 to perform Protein Expression Prokaryotic cells - E. coli D1

Get tips on using pAU5-amyE to perform Protein Expression Prokaryotic cells - C. glutamicum amyE

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.

Get tips on using pCEP-Pu to perform Protein Expression Eukaryotic cells - HEK293 Guinea Pig TNF-Alpha

Get tips on using pcDNA3.1-MRP4-his6 to perform Protein Expression Eukaryotic cells - HEK293 human MRP4-his6

Get tips on using psIL-15Rα/Fc to perform Protein Expression Eukaryotic cells - HEK293 sIL-15Rα/Fc

Get tips on using Live and Dead Cell Assay (Abcam) to perform Live / Dead assay mammalian cells - rabbit bone marrow mesenchymal stem cells