rna-isolation-purification-cells-primary-rat-brain-microvascular-endothelial-cells

Get tips on using Magnetic mRNA Isolation Kit to perform RNA isolation / purification Tissue - Rat Blood / Serum / Plasma / Buffy coat

Get tips on using PureLink™ RNA Mini Kit to perform RNA isolation / purification Cells - immortalized CAMA-1

Get tips on using AllPrep DNA/RNA Mini Kit to perform RNA isolation / purification Cells - immortalized Hepa-1c1c7

Get tips on using PureLink™ RNA Mini Kit to perform RNA isolation / purification Cells - immortalized SH-SY5Y

Get tips on using AllPrep DNA/RNA Mini Kit to perform RNA isolation / purification Cells - immortalized NK-92

Get tips on using PureLink™ RNA Mini Kit to perform RNA isolation / purification Cells - immortalized MCF-7

Get tips on using PureLink™ RNA Mini Kit to perform RNA isolation / purification Cells - immortalized MCF 10A

Get tips on using PureHelix™ RNA Extraction Solution to perform RNA isolation / purification Cells - immortalized HT-1080

Get tips on using PureLink™ RNA Mini Kit to perform RNA isolation / purification Cells - immortalized Caco-2

RNA-Seq is a method to sequence RNA by applying Next Generation Sequencing (NGS). The quality of RNA is critical for the success of RNA-Seq. The integrity of RNA is measured by the RNA integrity number (RIN). RIN is computed from RNA electrophoresis and electropherogram profiles (the peak area of the 28S rRNA should be approximately twice the peak area of the 18S rRNA). If you get the RIN value lower than 7, the possibility of getting the low quality of RNA-seq data is high. To get a high quality RNA, it is better to work with fresh samples or snap-freeze the tissues in liquid nitrogen as quickly as possible and store them at -80°C until further use. Make sure designated areas and all your filter tips, microfuge tubes, plastic, and glassware are RNase-free.