siRNA / miRNA gene silencing Human HeLa

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Proteins Protein expression and purification Mammalian cells HeLa ChaC1

Isolating DNA from tissues and paraffin-embedded tissue samples can be challenging as double-stranded DNA is physically fragile and highly susceptible to exo- and endonucleases. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in the presence of DNAse inhibitors. Further, extracting DNA from the nucleus need specific methods by combining physical, mechanical and chemical lysis approaches,

DNA DNA isolation / purification Cells Immortalized cell lines HeLa

Cellular assays Cell line authentication Cervix carcinoma cell line HeLa S3

RNA siRNA / RNAi /miRNA transfection Mouse Primary cortical and hippocampal cell

Get tips on using TaqMan® MicroRNA Reverse Transcription Kit to perform siRNA / miRNA gene silencing Mouse - Glomerular mesangial cells HIPK2 Polymer / Lipid delivery

Products Thermo Fisher Scientific TaqMan® MicroRNA Reverse Transcription Kit

Get tips on using SLC20A2 Human Gene Knockout Kit to perform CRISPR Human - Repression SLC20A2

Products OriGene SLC20A2 Human Gene Knockout Kit

Get tips on using FOXA2 Human Gene Knockout Kit to perform CRISPR Human - Repression FOXA2

Products OriGene FOXA2 Human Gene Knockout Kit

Get tips on using Glut1 siRNA and shRNA Plasmids (h) to perform siRNA / RNAi /miRNA transfection Human Cells - HT-1376 GLUT1

Products Santa Cruz Biotechnology Glut1 siRNA and shRNA Plasmids (h)

Get tips on using CD74 siRNA and shRNA Plasmids (h) to perform siRNA / RNAi /miRNA transfection Human Cells - HT-1376 CD74

Products Santa Cruz Biotechnology CD74 siRNA and shRNA Plasmids (h)

Get tips on using β-Galactosidase Reporter Gene Staining Kit to perform Reporter gene assay β-galactosidase substrates - HeLa cervical cancer cells

Products Sigma-Aldrich β-Galactosidase Reporter Gene Staining Kit

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