Get tips on using lentiCRISPR v2 to perform CRISPR Human - Repression miR-21
Get tips on using lenti dCAS-VP64_Blast to perform CRISPR Human - Activation ASCL1
Get tips on using lenti dCAS-VP64_Blast to perform CRISPR Human - Activation RhoGDIα
Get tips on using M-ST1n-VP64 to perform CRISPR Human - Activation RHOXF2
Get tips on using M-ST1n-VP64 to perform CRISPR Human - Activation MIAT
Get tips on using pSLQ1658-dCas9-EGFP to perform CRISPR Human - Activation BRCA1
Get tips on using pcDNA-dCas9-VP64 to perform CRISPR Human - Activation REPRIMO
Get tips on using Cas9 sgRNA vector to perform CRISPR Human - Activation ESR1
Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase
Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase
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