DNA transfection Mammalian cells Primary cells

Get tips on using RIPA Buffer (10X) to perform Protein isolation Mammalian cells - Human gingival epithelial cells

Get tips on using ISOGEN to perform RNA isolation / purification Cells - primary human epidermal melanocytes

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary mouse chondrocytes

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary rat osteoblasts

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary rat astrocytes

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary human preadipocytes

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary human chondrocytes

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary canine osteoblasts

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.