Protein expression and purification

Get tips on using M-PER™ Mammalian Protein Extraction Reagent to perform Protein isolation Tissue - Human aortic endothelial cells

Get tips on using pYT379-CDK8-CycC-10xHis complex to perform Protein Expression Eukaryotic cells - S. frugiperda CDK8-CycC-10xHis complex

Get tips on using pET30 Ek/LIC-Colα3(IV) NC1 to perform Protein Expression Prokaryotic cells - E. coli Colα3(IV) NC1

Get tips on using pFastBac1-A/reassortant/NYMC X-179-NP to perform Protein Expression Eukaryotic cells - S. frugiperda Influenza NP

Get tips on using Pierce™ Coomassie (Bradford) Protein Assay Kit to perform Protein quantification Mammalian cells - Human pluripotent stem cells

Get tips on using Pierce™ Cell Surface Protein Isolation Kit to perform Protein isolation Mammalian cells - Human aortic endothelial cells

Get tips on using M-PER™ Mammalian Protein Extraction Reagent to perform Protein isolation Mammalian cells - Human aortic endothelial cells

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.