Immunohistochemistry Anti-Glial Fibrillary Acidic Protein (GFAP) Mouse Human

Get tips on using Anti-ATG4B antibody produced in rabbit to perform Autophagy assay cell type - H4

Get tips on using Anti-trimethyl-Histone H3 (Lys9) Antibody to perform ChIP H3K9me3 - Sheep Rat -NA-

Get tips on using Anti-trimethyl-Histone H3 (Lys4) Antibody to perform ChIP H3K4Me3 - Sheep Rat -NA-

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.

Get tips on using Pierce™ Coomassie (Bradford) Protein Assay Kit to perform Protein quantification Mammalian cells - Human pluripotent stem cells

Get tips on using Pierce™ Cell Surface Protein Isolation Kit to perform Protein isolation Mammalian cells - Human aortic endothelial cells

Get tips on using M-PER™ Mammalian Protein Extraction Reagent to perform Protein isolation Mammalian cells - Human aortic endothelial cells

Get tips on using Recombinant Anti-FAK antibody [EP695Y] (ab40794) to perform Western blotting Focal adhesion Kinase (FAK)

Get tips on using Anti-LC3B antibody produced in rabbit to perform Autophagy assay cell type - RLE-6TN

Get tips on using Anti-ATG7 antibody produced in rabbit to perform Autophagy assay cell type - UMR-106