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shRNA gene silencing

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Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase

Cellular assays Reporter gene assay β-galactosidase substrates INS-1 832/13

Get tips on using Silencer® Select Negative Control No 1 siRNA to perform siRNA / miRNA gene silencing Mouse - siRNA negative control polymer / lipid

Products Thermo Fisher Scientific Silencer® Select Negative Control No 1 siRNA

Get tips on using ON-TARGETplus Human PPRC1 siRNA to perform siRNA / miRNA gene silencing Human - MCF-7 PRC (PGC-1α–related coactivator)/PPRC1

Products Horizon Discovery Ltd. ON-TARGETplus Human PPRC1 siRNA

Get tips on using ON-TARGETplus Human ITGB3 (3690) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - MDA-MB-231 β3 integrin/ITGB3

Products Horizon Discovery Ltd. ON-TARGETplus Human ITGB3 (3690) siRNA - SMARTpool

Get tips on using ON-TARGETplus Human ITGB1 (3688) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - MDA-MB-231 β1 integrin/ITGB1

Products Horizon Discovery Ltd. ON-TARGETplus Human ITGB1 (3688) siRNA - SMARTpool

Get tips on using ON-TARGETplus Human LIN28A (79727) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - hES cell line H1 (WA01) LIN28

Products Horizon Discovery Ltd. ON-TARGETplus Human LIN28A (79727) siRNA - SMARTpool

Get tips on using SignalSilence® NF-κB p65 siRNA I #6261 to perform siRNA / miRNA gene silencing Rat - H9c2 NF-κB RelA (p65)

Products Cell Signaling Technology SignalSilence® NF-κB p65 siRNA I #6261

DNA microarrays enable researchers to monitor the expression of thousands of genes simultaneously. However, the sensitivity, accuracy, specificity, and reproducibility are major challenges for this technology. Cross-hybridization, combination with splice variants, is a prime source for the discrepancies in differential gene expression calls among various microarray platforms. Removing (either from production or downstream bioinformatic analysis) and/or redesigning the microarray probes prone to cross-hybridization is a reasonable strategy to increase the hybridization specificity and hence, the accuracy of the microarray measurements.

DNA Microarray Gene expression arrays Rat mesothelium Satin cocktail

DNA microarrays enable researchers to monitor the expression of thousands of genes simultaneously. However, the sensitivity, accuracy, specificity, and reproducibility are major challenges for this technology. Cross-hybridization, combination with splice variants, is a prime source for the discrepancies in differential gene expression calls among various microarray platforms. Removing (either from production or downstream bioinformatic analysis) and/or redesigning the microarray probes prone to cross-hybridization is a reasonable strategy to increase the hybridization specificity and hence, the accuracy of the microarray measurements.

DNA Microarray Gene expression arrays Mouse dorsal skin Biotin

DNA microarrays enable researchers to monitor the expression of thousands of genes simultaneously. However, the sensitivity, accuracy, specificity, and reproducibility are major challenges for this technology. Cross-hybridization, combination with splice variants, is a prime source for the discrepancies in differential gene expression calls among various microarray platforms. Removing (either from production or downstream bioinformatic analysis) and/or redesigning the microarray probes prone to cross-hybridization is a reasonable strategy to increase the hybridization specificity and hence, the accuracy of the microarray measurements.

DNA Microarray Gene expression arrays Mouse brain tissue Biotin

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