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Found 2 matching solutions for this experiment
|Seed 1 × 10^5 cells/well
|Add 2 ml of 1X Fixing Buffer and incubate 5–10 minutes at room temperature.
Add 2 ml of the X-Gal Staining Mix to the dish and incubate the dish at 37°C for 1 hr to overnight
|Add 2 mL of 1X Fixing Solution. Incubate at room temperature for 5 minutes.
Wash cells and add Cell
Staining Working Solution.
Incubate the cells at 37ºC protected from light for 1 hr to overnight.
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