siRNA / miRNA gene silencing Mouse C2C12

Get tips on using Ovation® RNA-Seq System V2 to perform RNA sequencing Mouse - C2C12

Get tips on using GeneArt™ Site-Directed Mutagenesis System to perform Site Directed Mutagenesis (SDM) Mouse - C2C12 myogenin

The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

Get tips on using Luciferase Assay System to perform Reporter gene assay β-galactosidase substrates - C2C12

Get tips on using FxCycle™ PI/RNase Staining Solution to perform Cell cycle assay mouse - C2C12

Get tips on using SMS2 CRISPR/Cas9 KO Plasmid (m) to perform CRISPR Mouse - Deletion C2C12 Sgms2

Get tips on using SMS1 CRISPR/Cas9 KO Plasmid (m) to perform CRISPR Mouse - Deletion C2C12 Sgms1

Get tips on using X-Gal Staining Kit to perform Reporter gene assay β-galactosidase substrates - C2C12

Get tips on using GeneArt™ Site-Directed Mutagenesis System to perform Site Directed Mutagenesis (SDM) Mouse - Point mutation C2C12 myogenin