siRNA / miRNA gene silencing Mouse RGC-5

Get tips on using MethylFlash Methylated DNA 5-mC Quantification Kit to perform DNA quantification Human - HEK 293

Get tips on using MethylFlash Methylated DNA 5-mC Quantification Kit to perform DNA quantification Human - THP 1

Get tips on using QIAamp 96 DNA QIAcube HT Kit (5) to perform DNA isolation / purification Tissue - blood / plasma

Get tips on using QIAamp 96 PowerFecal QIAcube HT Kit (5) to perform DNA isolation / purification Micorbiome - Human gut

Get tips on using 5 µm Chemotaxis Assays, 24-Well Format to perform Cell migration / Invasion cell type - A549

Get tips on using QIAamp 96 Virus QIAcube HT Kit (5) to perform RNA isolation / purification Viral - SARS-CoV-2

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

Get tips on using BLUEstain™ 2 Protein ladder, 5-245 kDa to perform Protein Ladder Prestained

The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.

Cell cycle can be challenging due to difference introduced by sample handling, timing, and difference within the sample. Downstream instriuments to analyse cell cycle (Multicolor flow cytometry and multicolor imaging) can answer these challenges. Relevant markers can be combined with cell phenotyping markers to look at events within subpopulations of cells.