An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.
Get tips on using ApopTag® Fluorescein In Situ Apoptosis Detection Kit to perform TUNEL assay cell type - A549, NCI-H460, H1299 human lung cancer cells
Get tips on using FragEL™ DNA Fragmentation Detection Kit, Colorimetric - TdT Enzyme to perform TUNEL assay cell type - A127, U87MG, U251MG, T98G human glioblastoma cells
Get tips on using Comet Assay Kits, 96-Well to perform DNA Damage Assay SK-OV-3
Get tips on using Comet Assay Kits, 96-Well to perform DNA Damage Assay SK-OV-3
Get tips on using Comet Assay Kits, 96-Well to perform DNA Damage Assay MIA PaCa-2
Get tips on using Comet Assay Kits, 96-Well to perform DNA Damage Assay MDA-MB-231
Get tips on using Microbial Viability Assay Kit-WST to perform Live / Dead assay bacteria - Listeria innocua
Get tips on using Microbial Viability Assay Kit-WST to perform Live / Dead assay bacteria - Staphylococcus epidermidis
Get tips on using In Vitro Angiogenesis Assay Kit to perform Angiogenesis assay mouse - spleen-derived EPCs
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