Protein Expression Eukaryotic cells HeLa R19

The RNA interference (RNAi) is used to inhibit gene expression or translation, by neutralizing targeted mRNA molecules. Two types of RNA molecules such as microRNA (miRNA) and small interfering RNA (siRNA) play a central role in RNAi. Few points have to considered to increase the transfection efficiency of siRNA. Always use healthy, actively dividing cells to maximize transfection efficiency. The confluency of cells should be between 50-70%. Always use the most appropriate siRNA concentration to avoid off-target effects and unwanted toxic side effects. Positive and negative controls should be used for each and every experiment to determine transfection efficiency.

Get tips on using Cell Proliferation Kit I (MTT) to perform Cell cytotoxicity / Proliferation assay cell type - HeLa

Transfection is a powerful technique that enables the study of the function of genes and gene products in cells. Based on the nature of experiments, we may need a stable DNA transfection in cells for persistent gain-of-function or loss-of-function of the target gene. For stable transfection, integration of a DNA vector into the chromosome is crucial which requires selective screening and clonal isolation. By carefully selecting a viral delivery system and related reagents we can ensure safe and highly-efficient delivery of expression constructs for high-level constitutive or inducible expression in any mammalian cell type.

Get tips on using Alexa Fluor® 488 Annexin V/Dead Cell Apoptosis Kit to perform Necrosis HeLa

Get tips on using Anti-p62/SQSTM1 antibody produced in rabbit to perform Autophagy assay cell type - HeLa

Get tips on using Anti-SQSTM1 / p62 antibody [EPR4844] - Autophagosome Marker to perform Autophagy assay cell type - HeLa

Get tips on using BLOCK-iT™ Adenoviral RNAi Expression System, pAd/BLOCK-iT™-DEST RNAi Gateway Vector to perform shRNA gene silencing Mouse - P19 Foxm1

Get tips on using Color Prestained Protein Standard, Broad Range (10-250 kDa) to perform Protein Ladder Prestained

Get tips on using Blu13 (BLUelf) Prestained Protein Ladder（5 to 245 kDa） to perform Protein Ladder Prestained

Get tips on using Blu12 (BLUeye) Prestained Protein Ladder（11 to 245 kDa） to perform Protein Ladder Prestained