siRNA / miRNA gene silencing Rat RBL-2H3

Get tips on using Prolactin Rat ELISA to perform ELISA Rat - PRL

Get tips on using Rat Gastrin EIA to perform ELISA Rat - Gastrin

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.

Get tips on using Leptin (rat), ELISA kit to perform ELISA Rat - Leptin

Get tips on using Rat Leptin ELISA Kit to perform ELISA Rat - Leptin

Get tips on using Rat Gastrin ELISA Kit to perform ELISA Rat - Gastrin

Get tips on using BDNF Rat ELISA Kit to perform ELISA Rat - BDNF

Get tips on using Rat Activin-A ELISA to perform ELISA Rat - Activin

Get tips on using Rat Adiponectin ELISA Kit to perform ELISA Rat - Adiponectin

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.