siRNA / miRNA gene silencing Human BxPC-3

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Get tips on using QCM ECMatrix Cell Invasion Assay, 24-well (8 µm), fluorimetric to perform Cell migration / Invasion cell type - BxPC-3

Products Merck Millipore QCM ECMatrix Cell Invasion Assay, 24-well (8 µm), fluorimetric

Get tips on using Rock-2 siRNA and shRNA Plasmids (h) to perform siRNA / RNAi /miRNA transfection Human Cells - HT-1376 ROCK2

Products Santa Cruz Biotechnology Rock-2 siRNA and shRNA Plasmids (h)

Get tips on using CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS) to perform Cell cytotoxicity / Proliferation assay cell type - BxPC-3

Products Promega CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS)

Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase

Cellular assays Reporter gene assay luciferase human embryonic stem cells

Get tips on using APO-BrdU™ TUNEL Assay Kit, with Alexa Fluor™ 488 Anti-BrdU to perform Apoptosis assay cell type - BxPC-3

Products Thermo Fisher Scientific APO-BrdU™ TUNEL Assay Kit, with Alexa Fluor™ 488 Anti-BrdU

Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase

Cellular assays Reporter gene assay luciferase primary human endometrial stromal cells

Get tips on using miRNA Complete Labeling and Hyb Kit to perform Microarray RNA amplification & Labeling - Human endometrial stromal cells Cyanine 3-pCp

Products Agilent Technologies miRNA Complete Labeling and Hyb Kit

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

DNA Site Directed Mutagenesis (SDM) Human Deletion K562 c-Myb gene

Get tips on using 3-Methyladenine (3-MA) to perform Autophagy assay cell type - HL-1

Products Selleckchem.com 3-Methyladenine (3-MA)

Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase

Cellular assays Reporter gene assay luciferase HEK 293 human embryonic kidney cells

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