siRNA / miRNA gene silencing Human Jurkat MK2 (MAPK Kinase 2)

DNA microarrays enable researchers to monitor the expression of thousands of genes simultaneously. However, the sensitivity, accuracy, specificity, and reproducibility are major challenges for this technology. Cross-hybridization, combination with splice variants, is a prime source for the discrepancies in differential gene expression calls among various microarray platforms. Removing (either from production or downstream bioinformatic analysis) and/or redesigning the microarray probes prone to cross-hybridization is a reasonable strategy to increase the hybridization specificity and hence, the accuracy of the microarray measurements.

Get tips on using Bcl-2 Monoclonal Antibody (Bcl-2-100) to perform Western blotting Bcl- 2

Get tips on using SV Total RNA Isolation System to perform RNA isolation / purification Cells - immortalized Jurkat

Get tips on using FlowCellect Autophagy LC3 antibody based kit to perform Autophagy assay cell type - Jurkat

Get tips on using CYTO-ID® Autophagy detection kit to perform Autophagy assay cell type - Jurkat

Get tips on using pMIR-REPORT™ miRNA Expression Reporter Vector System to perform Reporter gene assay luciferase - HEK 293 human embryonic kidney cells

Get tips on using Rat Angiopoietin 2,ANG-2 ELISA Kit to perform ELISA Rat - ANGPT2

Get tips on using Lipofectamine® 2000 Transfection Reagent to perform siRNA / RNAi /miRNA transfection Mouse - Lung adenocarcenoma (A549/LTEP-a-2) Lipofectamine

Get tips on using EBMTM-2 Endothelial Cell Growth Basal Medium-2 to perform Mammalian cell culture media HCAEC

Get tips on using EGMTM-2 Endothelial Cell Growth Medium-2 BulletKit to perform Mammalian cell culture media RAOEC