siRNA / miRNA gene silencing Rat Astrocytes

Get tips on using Prolactin Rat ELISA to perform ELISA Rat - PRL

Get tips on using Rat Gastrin EIA to perform ELISA Rat - Gastrin

Get tips on using Leptin (rat), ELISA kit to perform ELISA Rat - Leptin

Get tips on using Rat Leptin ELISA Kit to perform ELISA Rat - Leptin

Get tips on using Rat Gastrin ELISA Kit to perform ELISA Rat - Gastrin

Get tips on using BDNF Rat ELISA Kit to perform ELISA Rat - BDNF

Get tips on using Rat Activin-A ELISA to perform ELISA Rat - Activin

Get tips on using Rat Adiponectin ELISA Kit to perform ELISA Rat - Adiponectin

Get tips on using Tf (Rat) ELISA Kit to perform ELISA Rat - Transferrin (Tf)

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.