Hello! I used Trizol to extract total RNA from in-vitro cultured bacteria (1 X 10^8 cells). After phase separation, I mixed ~0.4 ml of the upper phase which contains RNA with 0.5 mL cold isopropanol. However, the amount of RNA when measured in Nanodrop was very low. In addition, the ratio between 260 and 230 was around 0.1 to 0.5. Is there a chance that my sample was contaminated by the Trizol reagent? When I collected the aqueous phase I made sure to not touch the lower phase. What should I do?
Get tips on using miRNeasy Serum/Plasma Kit to perform RNA isolation / purification Tissue - Human Blood / Serum / Plasma / Buffy coat
Get tips on using miRNeasy Serum/Plasma Advanced Kit (50) to perform RNA isolation / purification Tissue - Livestock Blood / Serum / Plasma / Buffy coat
Get tips on using QIAamp DSP Virus Kit to perform DNA isolation / purification Tissue - blood / plasma
Get tips on using Gentra Puregene Blood Kit to perform DNA isolation / purification Tissue - blood / plasma
Get tips on using QIAamp 96 DNA Blood Kit to perform DNA isolation / purification Tissue - blood / plasma
Get tips on using QIAamp DNA Blood Midi Kit to perform DNA isolation / purification Tissue - blood / plasma
Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Tissue - Rat Blood / Serum / Plasma / Buffy coat
Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Tissue - Mouse Blood / serum / plasma / buffy coat
Get tips on using QIAamp MinElute ccfDNA Mini Kit (50) to perform DNA isolation / purification Tissue - blood / plasma
Fill out your contact details and receive price quotes in your Inbox
Outsource experiment