DNA Damage Assay Human bronchial epithelial cells (hBE)

Get tips on using FlexiGene DNA Kit (250) to perform DNA isolation / purification Tissue - FFPE samples

Get tips on using HotStarTaq DNA Polymerase (25000) to perform PCR Hot start PCR - Bacterial DNA

Get tips on using Yeast DNA Extraction Kit to perform DNA isolation / purification Yeast - Saccharomyces boulardii

Get tips on using HotStarTaq Plus DNA Polymerase to perform PCR Hot start PCR - Bacterial DNA

Get tips on using GoTaq® DNA Polymerase to perform PCR Conventional / Qualitative PCR - bacterial DNA

Get tips on using HotStarTaq Plus DNA Polymerase to perform PCR Conventional / Qualitative PCR - bacterial DNA

Get tips on using CpGenome Universal Methylated DNA to perform PCR Methylation specific PCR - Mammalian DNA

Get tips on using QIAamp DNA Mini Kit to perform DNA isolation / purification Yeast - Cryptococcus neoformans

Get tips on using QIAamp DNA Mini Kit to perform DNA isolation / purification Yeast - Candida albicans

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.