DNA transfection Mammalian cells

Get tips on using Glut1 siRNA and shRNA Plasmids (h) to perform siRNA / RNAi /miRNA transfection Human Cells - HT-1376 GLUT1

Get tips on using CD74 siRNA and shRNA Plasmids (h) to perform siRNA / RNAi /miRNA transfection Human Cells - HT-1376 CD74

Get tips on using CelLytic™ MT Cell Lysis Reagent to perform Protein isolation Mammalian cells - Human CD14+ cells

Get tips on using PowerSoil® DNA isolation - DNeasy PowerSoil Pro Kit to perform DNA isolation / purification Yeast - Cryptococcus neoformans

Get tips on using EZ DNA Methylation-Direct Kit to perform DNA methylation profiling Gene specific profiling - A2780 miR-30a-5p

Get tips on using EZ DNA Methylation-Direct Kit to perform DNA methylation profiling Gene specific profiling - A2780 miR-30c-5p

Get tips on using CpGenome Universal DNA Modification Kit to perform DNA methylation profiling Gene specific profiling - Ca Ski HPV-16

Get tips on using CpGenome Universal DNA Modification Kit to perform DNA methylation profiling Gene specific profiling - Human ovarian tissue TGFBI

The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.

Get tips on using PowerSoil® DNA isolation - DNeasy PowerSoil Pro Kit to perform DNA isolation / purification Bacteria - Gram negative Enterobacteriaceae