Protein Ladder

Get tips on using CelLytic™ B Cell Lysis Reagent to perform Protein isolation Bacteria - Salmonella enterica

Get tips on using CelLytic™ B Cell Lysis Reagent to perform Protein isolation Bacteria - Pseudomonas aeruginosa

Get tips on using CelLytic™ B Cell Lysis Reagent to perform Protein isolation Bacteria - Vibrio cholerae

Get tips on using CelLytic™ B Cell Lysis Reagent to perform Protein isolation Bacteria - Escherichia coli

Get tips on using CelLytic™ MT Cell Lysis Reagent to perform Protein isolation Tissue - Mouse heart

Get tips on using CelLytic™ MT Cell Lysis Reagent to perform Protein isolation Tissue - Mouse aorta

Get tips on using CelLytic™ MT Cell Lysis Reagent to perform Protein isolation Mammalian cells - HUVEC

Get tips on using CelLytic™ MT Cell Lysis Reagent to perform Protein isolation Mammalian cells - HeLa

In ChIP, the most vital step is the binding of an antibody and choosing the right antibody. The binding affinity of different types of immunoglobulins to protein A or G differs significantly. Henceforth, it is recommended to choose either protein A or protein G coated beads. If you do not see any product in the positive control, add 5–10 μg of chromatin and 1–5 μg of antibody to each IP reaction and incubate with antibody overnight and an additional 2 hr after adding Protein G/A beads. If no product in the experimental, add more DNA to the PCR reaction or increase the number of amplification cycles. Choose an alternate, ChIP-validated antibody if the antibody does not work.

In ChIP, the most vital step is the binding of an antibody and choosing the right antibody. The binding affinity of different types of immunoglobulins to protein A or G differs significantly. Henceforth, it is recommended to choose either protein A or protein G coated beads. If you do not see any product in the positive control, add 5–10 μg of chromatin and 1–5 μg of antibody to each IP reaction and incubate with antibody overnight and an additional 2 hr after adding Protein G/A beads. If no product in the experimental, add more DNA to the PCR reaction or increase the number of amplification cycles. Choose an alternate, ChIP-validated antibody if the antibody does not work.