TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.
Get tips on using OCT4-PG1 siRNA to perform siRNA / miRNA gene silencing Human - hES cell line H1 (WA01) OCT4-PG1
Get tips on using Attractene Transfection Reagent to perform shRNA gene silencing Human - HEK 293T CAPN5- (Calpains) cationic lipid based
Get tips on using mirVana™ miRNA Isolation Kit, with phenol to perform RNA isolation / purification Cells - primary human osteoblasts
Get tips on using Silencer select_ObR siRNA to perform siRNA / miRNA gene silencing Human - MCF-7 ObR(leptin receptor/LEPR)
Get tips on using Rock-2 siRNA and shRNA Plasmids (h) to perform siRNA / miRNA gene silencing Human - HT-1376 ROCK2
Get tips on using stealth siRNA GIRK1/KCNJ3 to perform siRNA / miRNA gene silencing Human - MDA-MB-453 GIRK1/KCNJ3
Get tips on using stealth siRNA GIRK1/KCNJ3 to perform siRNA / miRNA gene silencing Human - MDA-MB-231 GIRK1/KCNJ3
Fill out your contact details and receive price quotes in your Inbox
Outsource experiment