siRNA / RNAi /miRNA transfection Human Cells THP-1

Get tips on using jetPEI® DNA transfection, HTS application to perform DNA transfection Mammalian cells - Immortalized cell lines SH-SY5Y

An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

Get tips on using jetPEI® DNA transfection, HTS application to perform DNA transfection Mammalian cells - Immortalized cell lines MDA-MB-231

Get tips on using mirVana™ miRNA Isolation Kit, with phenol to perform RNA isolation / purification Cells - immortalized PK-1

Get tips on using Cell Death Detection ELISAPLUS to perform Apoptosis assay cell type - THP-1

Get tips on using Atg12 (D88H11) Rabbit mAb to perform Autophagy assay cell type - THP 1

Get tips on using Atg7 (D12B11) Rabbit mAb to perform Autophagy assay cell type - THP 1

Get tips on using Atg5 (D5F5U) Rabbit mAb to perform Autophagy assay cell type - THP 1

Get tips on using mirVana® miRNA mimic to perform RNA isolation / purification Cells - primary human endothelial cells

Get tips on using X-tremeGENE™ HP DNA Transfection Reagent to perform DNA transfection Mammalian cells - Immortalized cell lines HeLa