siRNA / miRNA gene silencing Human UCC (urothelial cancer cell lines)

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Isolating DNA from tissues and paraffin-embedded tissue samples can be challenging as double-stranded DNA is physically fragile and highly susceptible to exo- and endonucleases. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in the presence of DNAse inhibitors. Further, extracting DNA from the nucleus need specific methods by combining physical, mechanical and chemical lysis approaches,

DNA DNA isolation / purification Cells Immortalized cell lines Renal cortical tubule epithelial cells

Get tips on using TaqMan® MicroRNA Reverse Transcription Kit to perform siRNA / miRNA gene silencing Mouse - Glomerular mesangial cells HIPK2 Polymer / Lipid delivery

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Hello, can someone here help me? I am trying to silence e-selectin and ICAM-1 in endothelial cells. I would like to know if this is possible using shRNA

Discussions Multiple gene silencing using ShRNA

Get tips on using Galacto-Star™ β-Galactosidase Reporter Gene Assay System for Mammalian Cells to perform Reporter gene assay β-galactosidase substrates - MCF-7 human breast cancer

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Get tips on using Blood & Cell Culture DNA Midi Kit (25) to perform DNA isolation / purification Cells - Immortalized cell lines Human Neuroblastoma Cell Lines

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Get tips on using ICAfectin®442 siRNA transfection to perform DNA transfection Mammalian cells - Immortalized cell lines COS7

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Get tips on using ICAfectin®442 siRNA transfection to perform DNA transfection Mammalian cells - Immortalized cell lines HeLa

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Get tips on using IntestiCult™ Organoid Growth Medium (Human) to perform 3D Cell Culture Media Human gastric cancer organoids

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Get tips on using IntestiCult™ Organoid Growth Medium (Human) to perform 3D Cell Culture Media Human pancreatic cancer organoids

Products STEMCELL technologies IntestiCult™ Organoid Growth Medium (Human)

Get tips on using IntestiCult™ Organoid Growth Medium (Human) to perform 3D Cell Culture Media Human cancer colon organoids

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